A total of 93 ocular, 50 nasal swabs and 10 lung tissue were collected from 2-3 years old cattles affected with respiratory manifestation, and 145 serum samples from in contact apparently healthy cattles distributed in 4 different Governorates of Egypt (Ismalia, Qalioubia, Kafr El-Sheikh, and EL-Fayoum). Swabs and lung tissue samples were subjected to Bovine Herpes Virus (BHV-1) isolation on tissue culture Medin –Darby Bovine Kidney (MDBK) cell line and isolates were identified by direct fluorescent antibody technique (FAT) and polymerase chain reaction (PCR) by using primers complementary to the sequence of BHV-1 glycoprotein gB gene, as a rapid and more sensitive technique. From the results of virus isolation and identification were confirmed the more sensitivity of virus isolation than FAT and the most sensitivity and rapidity of PCR technique with confirmation and agreement of its results with the conventional methods. BHV-1 specific antibodies was detected in serum samples collected from the same 4 different Governorate, because of virus latency is a normal sequel to BHV-1 infection, by using the most suitable, sensitive, rapid, and specific techniques are ELISA and serum neutralization test (SNT), it is clear that the highest percentage of positive BHV-1 serum reactors was, in Kafr El-Sheikh followed by Ismalia, and Qalioubia and the lowest percentage was in EL-Fayoum. By the detection and identification of BHV-1 in collected samples and presence of positive reactors in serum samples this was a good indication for the circulation of BHV-1 in cattles of Egypt.