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112062

ISOLATION AND SEQUENCING OF MRF4 GENE (EXON 1 THROUGH 4) FROM EGYPTIAN BUFFALO

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

Muscle regulatory factor 4(MRF4, also known as herculin and MYF6) is a member of MyoD family, which is involved in the differentiation and maturation of myotubes and highly expressed postnatally.The sequences of this gene are well known in cattle and small ruminants but in buffalo no available sequence was previously recorded before this research. Therefore, the main aim of this study is to isolate and sequence exon 1 (E1) through exon 4 (E4) portion of this gene, which is the most appropriate portion of MRF4 that contains single nucleotide polymorphisms (SNPs), in buffalo. To achieve this, RT-PCR was performed in which gene-specific primers flanking to E1-E4 of MRF4 were utilized in addition to PCR master mix. A PCR product of 693bp, of MRF4 gene corresponding to the expected theoretical product size, was successfully amplified. Sequencing of the purified PCR product and its alignment against a previously known bovine gene identified 4 synonymous novel SNPs in buffalo MRF4. This result indicates that the sequence of MRF4 is highly conserved between cattle and buffalo. This is a preliminary study that provides the researchers withraw data which could be used as a basis for further studies to associate these SNPs with meat quality traits in buffalo.

DOI

10.21608/kvmj.2012.112062

Keywords

muscle regulatory factor 4, Sequencing, RT-PCR

Authors

First Name

H.E.

Last Name

Abbas

MiddleName

-

Affiliation

Department of biochemistry, Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.

Email

-

City

-

Orcid

-

First Name

A.M.

Last Name

El- kattawy,

MiddleName

-

Affiliation

Department of biochemistry, Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.

Email

-

City

-

Orcid

-

First Name

A. A.M

Last Name

Mokhbatly,

MiddleName

-

Affiliation

Department of clinical pathology, Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.

Email

-

City

-

Orcid

-

First Name

M.E.R.

Last Name

Abu El-Magd

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.

Email

-

City

-

Orcid

-

Volume

10

Article Issue

2

Related Issue

16743

Issue Date

2012-10-01

Receive Date

2012-07-01

Publish Date

2012-10-01

Page Start

57

Page End

67

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 10_Issue 2_Pages 57-67.pdf

PDF . 813.4kB

Link

https://kvmj.journals.ekb.eg/article_112062.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=112062

Order

4

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

ISOLATION AND SEQUENCING OF MRF4 GENE (EXON 1 THROUGH 4) FROM EGYPTIAN BUFFALO

Details

Type

Article

Created At

22 Jan 2023