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110201

INFLUENCE OF GLUTATHIONE ADDITION TO THE EXTENDER ON THE COOLED CAMEL SEMEN QUALITY

Article

Last updated: 04 Jan 2025

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Tags

Veterinary Surgery and Theriogenology.

Abstract

Semen was collected, evaluated and extended with two extenders (Lactose-yolk-citrate: LYC and Tris-yolk-fructose: TYF .The final extension rate was 1ml semen:4ml extender. The extended semen was divided into cervical 4 tubes added with Glutathion (GSH) at concentrations of 0.0, 0.2, 0.4 and 0.8 mM/100ml. The extended semen was kept at 5 ºC for5 days. After each storage time (0, 1, 2,3, 4 and 5 days), the percentages of sperm motility, dead spermatozoa, abnormal spermatozoa, and acrosome damage of spermatozoa were recorded. Activities of aspartate-aminotransferase (AST) and alanine-aminotrans ferase (ALT) enzymes were recorded. The penetrating ability of camel spermatozoa added with 0.4 mM into she-camel cervical mucus, during incubation at 37ºC for up to 4 hours was also recorded.
The results showed that, the extended cooled camel semen with TYF extender was insignificantly higher the percentage of motile spermatozoa, while insignificantly lower the percentages of dead spermatozoa, abnormal spermatozoa and acrosome damage and leakage of AST and ALT enzymes into the extra cellular medium than LYC extender, during storage at 5ºC for 5 days with the different concentrations of glutathione (GSH) or free – GSH medium. The advancement of storage time at 5ºC for up to 5days was significantly (P<0.05) decreased the percentage of sperm motility , while increased significantly ((P<0.05) the percentages of dead spermatozoa, abnormal spermatozoa, acrosome damage and enzymatic activities(AST and ALT) with different extenders or GSH concentrations. The penetrating ability of the extended camel spermatozoa with TYF or LYC extender added with 0.4 mM/100 ml GSH into she–camel cervical mucus was significantly (P<0.05)  better than free-GSH medium (control), during incubation at 37 ºC for up to 4 hours. However, the advancement of incubation time at 37ºC decreased significantly (P<0.05) the penetration score.

DOI

10.21608/kvmj.2019.110201

Keywords

Glutathion, Extender, camel semen quality

Authors

First Name

A. E. B.

Last Name

Zeidan

MiddleName

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Affiliation

Animal Production Research Institute, Dokki, Giza, Egypt.

Email

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City

-

Orcid

-

First Name

A. M.

Last Name

El-Sharabassy

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Y. A.

Last Name

Dowidar

MiddleName

-

Affiliation

Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

Ashour

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Email

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City

-

Orcid

-

First Name

A. M.

Last Name

Amer

MiddleName

-

Affiliation

Animal Production Research Institute, Dokki, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

E. A. A.

Last Name

Ahmadi

MiddleName

-

Affiliation

Animal Production Research Institute, Dokki, Giza, Egypt.

Email

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City

-

Orcid

-

First Name

R. M.

Last Name

Abou-Aiana

MiddleName

-

Affiliation

Animal Production Research Institute, Dokki, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

M. H.

Last Name

Farouk

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

M. H.

Last Name

Arafat

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

Volume

17

Article Issue

1

Related Issue

16741

Issue Date

2019-04-01

Receive Date

2019-01-01

Publish Date

2019-04-01

Page Start

37

Page End

61

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 17_Issue 1_Pages 37-61.pdf

PDF . 887.8kB

Link

https://kvmj.journals.ekb.eg/article_110201.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110201

Order

3

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

INFLUENCE OF GLUTATHIONE ADDITION TO THE EXTENDER ON THE COOLED CAMEL SEMEN QUALITY

Details

Type

Article

Created At

22 Jan 2023