This study was conducted to determine the distribution of E. coli in visceral organs from apparently healthy broiler chickens, diseased broiler chickens and freshly dead ones were collected from Kafr-Elsheik Governorate. A total number of 320 chicken organs in winter season and 300 chicken organs in summer season consisting of (80 and 75) from liver, (80 and75) from heart blood, (80 and 75) from spleen and (80 and 75) from kidney in winter and summer  seasons , respectively.Samples were aseptically collected from suspected  colibacillosis and subjected to bacteriological and biochemical examination.Processed tissue from each sample was inoculated on nutrient broth and incubated aerobically at 37ºC for 12 hours. A loopful from incubated nutrient broth was cultured primarily on MacConkey's agar plates.  Positive colonies (pink colonies) were picked up and recultivated on EMB at 37°C for 24 hrs. Presumptive colonies of bacterial agents on media plates were subjected to conventional biochemical characterization.  The result of biochemical test identified the E. coli was recovered from 172chicken organs with an incidence rate (27.74%), the high incidence of E.coli was recovered from liver (50% and 34.6%),followed by fresh heart blood (38.7% and 32%), spleen (22.5% and12%) and kidneys (18.7% and 12%) in winter and summer  seasons , respectively.