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110174

PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

Bovine ephemeral fever(BEF) suspected outbreaks recorded during the summer of (2013 to 2016)in the Delta provinces of Egypt. The aim of this study was the isolation and molecular characterization of BEF viruses detected from these outbreaks, as there is no currently information about BEFV in Egypt. Blood samples were collected from suspected cases andthe virus was isolated intracerebrallyin baby mice then passed to Madin Darby Bovine Kidney (MDBK) andAfrican green monkey kidney (VERO) cell lines. The isolated virus was identified by RT-PCR followed by sequencing of the glycoprotein (G) gene of BEFV isolate.The construction of phylogenetic tree based on genetic relationships of the BEFV isolate derived from Egypt and different countries was performed. The phylogenetic treeshowed that the Egyptian isolate was closely linked to the Israeli and Turkish isolates.
 

DOI

10.21608/kvmj.2018.110174

Keywords

BEFV, Glycoprotein G gene, RT-PCR, Sequencing, Egypt

Authors

First Name

Samy

Last Name

Kasem

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

samykasem2@gmail.com

City

-

Orcid

-

First Name

Noura

Last Name

Alkhalefa

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

Asmaa

Last Name

Magouz

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

Basma

Last Name

Ebrahem

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Khodier

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Walied

Last Name

Abdo

MiddleName

-

Affiliation

Department of pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

waliedsobhy@yahoo.com

City

-

Orcid

-

First Name

Abdelrazek

Last Name

Desouky

MiddleName

-

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

Volume

16

Article Issue

1

Related Issue

16723

Issue Date

2018-04-01

Receive Date

2018-02-20

Publish Date

2018-04-16

Page Start

1

Page End

15

Print ISSN

1687-1456

Online ISSN

2682-2954

Link

https://kvmj.journals.ekb.eg/article_110174.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110174

Order

1

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)

Details

Type

Article

Created At

22 Jan 2023