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110208

MOLECULAR CHARACTERIZATION OF EQUINE HERPES VIRUS -1 (EHV-1) IN EGYPT

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

Equine Herpes Virus -1 is one of the most viruses affecting equide family(Horse, Donkey &Mule) causing severe economic losses to horse industry all over the world, due to respiratory manifestations, abortion of pregnant mares ,and mayeloencephalopathy. Little reports have been made for investigation the prevalence and existence of equine herpes viruses (EHVs) in Egypt. In the present study, seventy clinical samples (6 placentas & 64 nasal swabs) collected from different governorate in Egypt. Virus isolation by inoculating tissue suspensions into MDBK cells revealed characteristic CPE for EHV after the fourth passage within 7 days from inoculation in the form of aggregation of the cells together, rounding, and finally cell detachment. Serological confirmation of the isolated sample by FAT revealed intracytoplasmic greenish yellow fluorescence. DNA extraction and PCR of suspected samples existed Successful amplification of 700 bp of UL45 gene of EHV-1 in 8 out of 70 examined samples.

DOI

10.21608/kvmj.2018.110208

Keywords

equine, Egypt, EHV-1, virus isolation, FAT and PCR

Authors

First Name

Noura .F.

Last Name

Alkhalefa

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Email

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City

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Orcid

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First Name

Ismail .I.

Last Name

Elkon

MiddleName

-

Affiliation

Department of Gynecology &Artificial Insemination, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

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First Name

Neven .A.

Last Name

Tolba

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

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City

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Orcid

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First Name

Effat .L.

Last Name

Elsayed

MiddleName

-

Affiliation

Rinder pest like disease department, Veterinary Serum & Vaccine Research Institute –Abasia –Cairo.

Email

-

City

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Orcid

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Volume

16

Article Issue

2

Related Issue

16720

Issue Date

2018-10-01

Receive Date

2018-07-01

Publish Date

2018-10-01

Page Start

61

Page End

73

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 16_Issue 2_Pages 61-73.pdf

PDF . 403.4kB

Link

https://kvmj.journals.ekb.eg/article_110208.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110208

Order

4

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF EQUINE HERPES VIRUS -1 (EHV-1) IN EGYPT

Details

Type

Article

Created At

22 Jan 2023