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Thymoquinone attenuates dimethoate induced hepatic and testicular genotoxicity in rats

Article

Last updated: 25 Dec 2024

Subjects

-

Tags

Veterinary Anatomy, Histology and Physiology “Veterinary Academic Studies".

Abstract

Background: Dimethoate (DM) is an organophosphate insecticide widely used on crops in Egyptian fields. DM has many toxic impacts on non-target organisms especially mammals.
Objective: This study aimed to evaluate therole of thymoquinone (TQ) against DM-induced hepatic and testicular subchronic genotoxicity.
Methods: Forty- eight rats were randomly divided into 6 groups each of eight animals. Group 1 served as the normal control received distilled water; Group 2 was given DM; Group 3 received DM+TQ; Group 4 was treated by TQ; Group 5 was supplemented with DM+ olive oil (as a vehicle); and Group 6 was given olive oil. Oxidative/antioxidant status was assessed by quantification of malondialdehyde (MDA) levels and superoxide dismutase (SOD) and catalase (CAT) activities. Genotoxicity and apoptosis in the liver and testis were evaluated using the comet assay and immunohistochemical expression of caspase3 and Bax, respectively.
Results: Significant DNA damage, marked histopathological changes in liver and testis, intense nuclear and cytoplasmic immune-expression of caspase 3 and Bax, significant decreases in catalase (CAT) and superoxide dismutase (SOD) activities and significant increase in malondialdehyde (MDA) levels were observed in DM-treated rats as compared to the control group. Concomitant treatment of TQ with DM drastically alleviated all the above-mentioned alterations.
Conclusion: These findings suggest that TQ exhibits an outstanding ameliorative role against DM-induced hepatic and testicular genotoxicity in rats through the mitigation of oxidative stress and apoptosis.

DOI

10.21608/kvmj.2020.40514.1010

Keywords

apoptosis, dimethoate, genotoxicity, Oxidative Stress, Thymoquinone

Authors

First Name

Fatma

Last Name

Zakzook

MiddleName

-

Affiliation

Department of Forensic Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

fatmablal2@gmail.com

City

-

Orcid

-

First Name

Hanaa

Last Name

Hegazy

MiddleName

-

Affiliation

Department of Forensic Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

drhantox@yahoo.com

City

-

Orcid

-

First Name

Tarek

Last Name

Yosef

MiddleName

-

Affiliation

Department of Forensic Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

tarekyosef70@yahoo.com

City

-

Orcid

-

First Name

Ghada

Last Name

Gomaa

MiddleName

-

Affiliation

Department of Forensic Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

ghadaagiz2000@yahoo.com

City

-

Orcid

-

Volume

18

Article Issue

2

Related Issue

16719

Issue Date

2020-10-01

Receive Date

2020-08-25

Publish Date

2020-10-30

Page Start

25

Page End

32

Print ISSN

1687-1456

Online ISSN

2682-2954

Link

https://kvmj.journals.ekb.eg/article_120820.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=120820

Order

6

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

Thymoquinone attenuates dimethoate induced hepatic and testicular genotoxicity in rats

Details

Type

Article

Created At

22 Jan 2023