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114218

GENOMIC RELATEDNESS OF TURKEY CORONAVIRUSES, BOVINE CORONAVIRUS AND INFECTIOUS BRONCHITIS VIRUS OF CHICKENS

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

The discrepant findings of antigenic relationship of turkey coronavirus (TCV) to infectious bronchitis virus (IBV) and bovine coronavirus (BCV) lead us to study the genomic relatedness of TCVs to IBV and BCV. A one step reverse transcription (RT)-polymerase chain reaction (PCR) targeting a 525-base pair fragment of the spike 2 (S2) gene of BCV was used in a trial to detect TCVs isolated from diarrheic turkeys and a prototype TCV obtained from the American Type Culture Collection. The one-step RT-PCR amplified cell culture-passaged isolates of calf diarrhea strains of BCV, but none of the tested TCVs. On the other hand when we used primers designed from S2 gene of IBV, The same TCVs could be detected using the S2 IBV primers of IBV of chickens which amplified a 820-base pair region spanning portions of the S2 glycoprotein genes of IBV and TCV. These findings provide evidence that TCV is more closely related to IBV.

DOI

10.21608/kvmj.2006.114218

Keywords

turkey coronavirus, bovine coronavirus, Infectious bronchitis virus, genomic relatedness

Authors

First Name

M. M.

Last Name

Ismail

MiddleName

-

Affiliation

Dept. of Poultry and Fish, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

A. Y.

Last Name

Metwally

MiddleName

-

Affiliation

Animal Health Research Institute, Kafr El-Sheikh Prov. Laboratory

Email

-

City

-

Orcid

-

Volume

4

Article Issue

2

Related Issue

16573

Issue Date

2006-10-01

Receive Date

2006-07-01

Publish Date

2006-10-01

Page Start

105

Page End

118

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 4_Issue 2_Pages 105-118.pdf

PDF . 616.5kB

Link

https://kvmj.journals.ekb.eg/article_114218.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=114218

Order

8

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

GENOMIC RELATEDNESS OF TURKEY CORONAVIRUSES, BOVINE CORONAVIRUS AND INFECTIOUS BRONCHITIS VIRUS OF CHICKENS

Details

Type

Article

Created At

22 Jan 2023