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112422

THE EFFECT OF SERUM STORAGE FACTORS INFLUENCING THE REACTIVITY OF ANTIBODIES IN SERODIAGNOSTIC TESTS

Article

Last updated: 04 Jan 2025

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Veterinary Microbiology and Parasitology.

Abstract

To investigatetheeffectofdifferentserumstoragemethodsonreactivity of bovineBrucellaantibodiesinserodiagnostictests,sera were collected fromBrucellainfected cattleandstoredeitherin a refrigerator at 4 ◦Cor in a deep freezer at   - 20 ◦C mean while reference serum samples were stored at 4 ◦C, - 20 ◦C and - 80 ◦C.
Monitoring of the Brucella antibody activity in stored samples, was performed by Slow Agglutination Test (SAT) on monthly basis for sixmonths and results obtained were analyzed in three dimensions. First, betweendifferenttimeintervals,second,betweendifferentstoragetemp-eratures and finally between collected sera and reference sera samples.
AnalysisoftheresultsrevealedasignificantreductionintiterofBrucella antibodies in sera stored at- 20 ◦C after two months of storage,whereas no significant difference could be detected in antibodies titer for up to four months of serum storage at 4 ◦C, meanwhile reference samples showed stability in titer of Brucella antibodies when compared with collected serum under the same storage temperatures.It was concluded thattheeffectof storagetemperature is more or less affected by quality of serum.
Since high quality serum is the cornerstone in sero-epidemiological studies and diagnosis, we recommended the necessity of sterilization of serum by filtration. Additionally, the constancy of cooling tempera-tureandtheavoidance of refreezing and thawing during storage should be considered in order to get accurate results from serum samples.

DOI

10.21608/kvmj.2004.112422

Keywords

serum storage methods, reactivity of bovine Brucella antibodies

Authors

First Name

Alaa El-Din

Last Name

Hussein, M.

MiddleName

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Affiliation

Department of Microbiology Faculty of Veterinary Medicine, Kafr El-Sheikh Tanta University

Email

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City

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Orcid

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First Name

Kamel M.

Last Name

Ammar

MiddleName

-

Affiliation

Department of Animal Medicine Faculty of Veterinary Medicine, Kafr El-Sheikh Tanta University

Email

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City

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Orcid

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Volume

2

Article Issue

2

Related Issue

16569

Issue Date

2004-10-01

Receive Date

2004-07-01

Publish Date

2004-10-01

Page Start

29

Page End

42

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 2_Issue 2_Pages 29-42.pdf

PDF . 711.5kB

Link

https://kvmj.journals.ekb.eg/article_112422.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=112422

Order

3

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

THE EFFECT OF SERUM STORAGE FACTORS INFLUENCING THE REACTIVITY OF ANTIBODIES IN SERODIAGNOSTIC TESTS

Details

Type

Article

Created At

22 Jan 2023