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112225

INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary Surgery and Theriogenology.

Abstract

The aim of the present study was to investigate the effect of follicle size on the buffalo cumulus oocyte complexes (COCs) morphology, chromatin configuration, level of apoptosis in cumulus cells, and concentration of progesterone and estradiol-17b in buffalo follicular fluid of different follicle size. Visible follicles were carefully isolated and classified according to their size into 3 categories  5 mm (5-8 mm). A total of 295 COCs were recovered from the previous three follicle categories; COCs were classified according to number and compactness of cumulus layers. Good quality compact COCs which used for IVM, which mostly obtained from follicles
The chromatin configuration at the time of recovery in oocytes derived from 3-5 mm follicle respectively.
  There was a connection of cumulus morphology, chromatin configuration at the time of recovery and the follicle size.On the other hand no significant differences in maturation capacity (metaphase2)of COCs derived from different follicles diameter.
Apoptosis of cumulus at time of recovery decreased with incre-asing the follicle size, but this difference was not significant (0.9, 0.6,0.2). After 24 hr. IVM, an increase in apoptosis was observed in cumulus cells from all follicle size with significant (P < 0.05) increase in cumuli derived from 3-5 mm follicles compared to < 3 and >5 mm follicles (24.4 Vs 4.7,3.5)
Concentration of progesterone (ng/ml)in buffalo follicular fluid was not influenced by follicle size, while concentration of estradiol-17b (ng/ml) was significant higher in follicle 3-5, >5 mm (782 ± 67 and 788 ± 67 respectively) in comparison to follicular fluid from

DOI

10.21608/kvmj.2003.112225

Keywords

Buffalo, oocyte, follicle size, apoptosis, progesterone, estradiol-17

Authors

First Name

B.

Last Name

Heleil

MiddleName

-

Affiliation

Dept. of Theriogenology, Faculty of Veterinary Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

H.

Last Name

Torner

MiddleName

-

Affiliation

Research Instit.Biology of Farm Animals, 18196 Dummerstorf, Germany.

Email

-

City

-

Orcid

-

First Name

Sh.A.

Last Name

Mahmoud,

MiddleName

-

Affiliation

Dept. of Physiology, Faculty of Veterinary Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

H.

Last Name

Alm

MiddleName

-

Affiliation

Research Instit.Biology of Farm Animals, 18196 Dummerstorf, Germany

Email

-

City

-

Orcid

-

First Name

E.M

Last Name

Fattouh

MiddleName

-

Affiliation

Dept. of Theriogenology, Faculty of Veterinary Medicine, Tanta University

Email

-

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

16568

Issue Date

2003-08-01

Receive Date

2003-01-01

Publish Date

2003-04-01

Page Start

345

Page End

362

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 1_Issue 1_Pages 345-362.pdf

PDF . 819.0kB

Link

https://kvmj.journals.ekb.eg/article_112225.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=112225

Order

6

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO

Details

Type

Article

Created At

22 Jan 2023