The present study was carried out to evaluate the correlation between the excretion of aflatoxin B1 residues in eggs of laying hens fed aflatoxin B1-contaminated ration and excretion of trichlorfon pesticide after farm spray with trichlorfon pesticide. 7 egg samples were collected daily for 7 days from laying hens fed on ration contained 15 ppb aflatoxin B1 within, from farm previously sprayed with trichlorfon pesticide (0.15%) for eradication of ticks. In this work, number and percentage of eggs contained aflatoxin B1 increased was increasing with time in both albumin and yolk till reached 100% after 3 days of hens trichlorfon exposure. Vice versa, number and percentage of eggs contained trichlorfon residues were higher directly after spraying then completely disappeared from yolk and albumin after 7 days. Aflatoxin B1 residues significantly increased comparing with time in albumin and insignificantly elevated in yolk, allover the experimental period. Otherwise, trichlorfon residues significantly dropped in both albumin and yolk till became undetectable at the 7th day. This study indicated that there are significant correlation between aflatoxin B1 and trichlorfon residues in albumin and yolk of tested egg samples. These results indicated the emphasizing importance of controlling aflatoxin levels in rations for laying hens especially when using pesticide for controlling ticks. Also, the withdrawal time for trichlorfon in eggs was observed to be 6 days. In addition, health significance about aflatoxin B1 and trichlorfon residues in egg have been discussed.