Twenty mature pregnant New Zealand rabbit were used to clarify the blood biochemical changes in lipids and fatty acid profile during pregnancy. Blood samples were obtained 0,10,20 and 30 days following pregnancy. Sera were collected and stored frozen at –20°C until determination of total lipid, triacylglycerol, cholesterol, phospholipids, HDL-C, LDL-C and fatty acids pattern. The obtained results revealed that the serum total lipid, triacylglycerol, cholesterol and phospholipids showed a significant decrease till parturition time reaches.  Both HDL-C & LDL-C were also significantly decreased along the period of pregnancy and finally there's some fatty acids patterns as laurate, palmitic, linoleic, linolenic, exhibited a significant increase through the pregnancy period where the myristate showed non-significant change and the arachidonic acid showed a highly significant increase throughout the pregnancy period.