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113575

MOLECULAR COMPARATIVE STUDY ON DIFFERENT STRAINS OF EQUINE HERPESVIRUS TYPE 1 (EHV-1) BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)

Article

Last updated: 04 Jan 2025

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Subjects

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Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

RacL11 and Kentucky D are classical virulent strains of equine hepesvirus-1 (EHV-1) which are worldwide used for vaccine manufacture. These two strains are the parent strains of commercial vaccines. The present study were applied in the Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, ,Japan in 2006. We compared the two strains with the reference strain Ab4P using Long and accurate PCR (LA-PCR) Restriction Fragment Length Polymorphism (RFLP) for the EHV-1 whole genomic DNA using eight different types of restriction enzymes (SacI, SspI, NaeI, ApaI, EcoRI, HincII, BglII, and StuI) which digest the whole genome into 600 fragments. Digests were electrophoresed on an agarose gel of the appropriate concentration. Restriction maps of RacL11 and Kentucky D genomic DNA in comparison to Ab4p revealed no restriction sites differences except in one fragment (Fragment 12) which corresponds to nt 104,320 to 111,287 in the genome sequence of the standard Ab4p strain and includes a part of ORF62, the intergenic region between ORFs 62-63, and a portion of ORF63.   and by sequencing we found that the intergenic region between ORFs  62-63 (IGR) revealed that the RacL11 and Kentucky D strains show sequence length difference between all examined strains. The increased length of fragment 12 of RacL11 was found to be due to the presence of 10 copies of 5'-GGCTAGCGCTAACGCTAG-3' while the Kentucky D and Ab4p sequences contained 4 copies and 3 copies, respectively. Using more restriction enzymes for future RFLP comparison is recommended.

DOI

10.21608/kvmj.2010.113575

Keywords

EHV-1, RFLP, DNA, Genomic differences

Authors

First Name

Yasser

Last Name

Ghanem

MiddleName

Mohamed

Affiliation

Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Egypt

Email

-

City

-

Orcid

-

First Name

Tomio

Last Name

Matsumura

MiddleName

-

Affiliation

Epizootic Research Station, Equine Research Institute, The Japanese Racing Association, Tochigi 329-04, Japan.

Email

-

City

-

Orcid

-

First Name

Hideto

Last Name

Fukushi

MiddleName

-

Affiliation

Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

Email

-

City

-

Orcid

-

Volume

8

Article Issue

1

Related Issue

16460

Issue Date

2010-04-01

Receive Date

2010-01-01

Publish Date

2010-04-01

Page Start

118

Page End

132

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 8_Issue 1_Pages 118-132.pdf

PDF . 757.0kB

Link

https://kvmj.journals.ekb.eg/article_113575.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=113575

Order

7

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR COMPARATIVE STUDY ON DIFFERENT STRAINS OF EQUINE HERPESVIRUS TYPE 1 (EHV-1) BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)

Details

Type

Article

Created At

22 Jan 2023