Subjects
-Tags
Veterinary Pharmacology, Toxicology and Forensic Medicine.
Abstract
30 fattening calves1 year old were divided into 3 groups (10 in each), 1st group injected with B. bigemina exoantigen vaccine (240 mg of total protein combined with 2 mg saponin/dose) 2 doses with one month interval then Inoculated with 1×109 of Babesia bigemina infected RBCs after 90 days of 2nd dose of vaccine, 2nd group injected with B. bigemina exoantigen vaccine 2 doses with one month interval, while 3rd group injected with 2 mg saponin 2 doses with one month interval then Inoculated with 1×109 of Babesia bigemina infected RBCs after 90 days of 2nd dose of vaccine. Significant increase in antibody titers against B. bigemina at day 21 after 1st and 2nd dose of vaccination while no antibodies were detected in the non vaccinated groupas measured by ELISA. Lymphocyte transformation index at day 15 after 1st and 2nd dose of vaccination was higher in vaccinated than non vaccinated animals. Immunized cattle with B. bigemina exoantigen vaccine were protected against virulent homologous challenge with 109 virulent b. bigemina infected RBCs and elicited higher antibody titer without showing any clinical signs when compared with control animals.
DOI
10.21608/kvmj.2011.113535
Keywords
Babesia, Babesia bigemina, Exoantigen, ELISA, cattle
Authors
MiddleName
-Affiliation
Lecturer of infectious diseases, Department of Animal Medicine, Faculty of
Veterinary Medicine, Alexandria University.
Email
-City
-Orcid
-Link
https://kvmj.journals.ekb.eg/article_113535.html
Detail API
https://kvmj.journals.ekb.eg/service?article_code=113535
Publication Title
Kafrelsheikh Veterinary Medical Journal
Publication Link
https://kvmj.journals.ekb.eg/
MainTitle
EFFICACY OF BABESIA BIGEMINA EXOANTIGEN VACCINE FOR PROTECTION AGAINST BABESIA BIGEMINA INFECTION