A total of 100 ducks having a history of respiratory disorders and mortality, in addition, 20 water and sediments samples each were examined in this study. Samples were collected from different private duck farms in a Sharkia Governorate for clinical, P.M and bacteriological examinations. Clinical signs of living ducks showed exhausted birds, loss of appetite, respiratory disorder (difficult breathing-coughing and watery nasal discharge), cyanosis of comb and wattles which were swollen and edematous, and watery green yellowish diarrhea. Postmortem examination revealed pneumonia, hemorrhages of various sizes in the heart, liver, and intestine. Also, congestion of the liver with necrotic foci. Bacteriological examination of these samples for the prevalence of Pasteurella spp. according to morphological characters and biochemical reactions , revealed isolation of 26 isolates of Pasteurella multocida { 21 isolates from ducks at a rate of 21%, 4 isolates from water samples at a rate of 20% and 2 isolates from sediments samples at a rate of 10% }. In addition , isolation of pasteurella haemolytica at a rate of 9 %. Sensitivity test revealed that pasteurella multocida were sensitive to Ceftifur, Enrofloxacin and followed by oxtetracycline, Erythromycin, Doxycycline and Trimethoprim-sulphmethoxazole. Meanwhile, all isolates were resistant to Neomycin, Chloromephanicol, Flumequine and Streptomycin. Results of experimental infection with pasteurella multocida with trials for treatment showed complete disappearance of clinical signs in ducks when treated with Enrofloxacin and Ceftiofur, also, reduced mortality to 24% and 16% for Ceftiofur and Enrofloxacin respectively. In the same time, showed significant increase in body weight and weight gain when compared with infected non treated ducks.