Beta
ManageSearchChatDiscover
110132

ASSOCIATION BETWEEN A31GSNP OF THE MYOSTATIN GENE AND SERUM LEVELS OF ALP, GOT, P AND CA IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

Myostatin, a member of the transforming growth factor ß (TGF-ß) superfamily, is a negative regulator of skeletal muscle growth in mammals. The loss or inhibition of myostatin signaling dramatically increases muscle mass. This study is aimed to detect single nucleotide polymorphisms (SNPs) in the myostatin (Mstn) gene and to associate measure their effect on mineral (Ca and P) and enzymatic (GOT and ALP) serum levels in high and low meat producing Egyptian buffaloes. Mstn gene was amplified by PCR and subsequently, subjected to sequence analysis to identify different allelic patterns. The nucleotide sequence analyses revealed presence of a novel A31GSNP in Mstn1 locus at nucleotide number 50 before exon2. This SNP was synonymous as it is located in the non coding sequence and so no amino acid was changed. However, the sequences of the other three loci showed 100% identity among the Egyptian buffaloes and as compare to the published sequences of buffalo (GenBank accession number, DQ091762). To identify whether A31G SNP is accompanied by change in serum levels of GOT, ALP enzymes, and Ca and Ph, the levels of these enzymes and minerals were estimated in the serum of high and low meat producing Egyptian buffaloes at age of 2 year. The serum levels of ALP, GOT, P, and Ca were significantly increased in high meat producing buffaloes as compared to low meat producing animals. The results of this study can be enable breeders to do an accurate and earlier selection of high meat producing buffaloes on a genotype basis.

DOI

10.21608/kvmj.2014.110132

Keywords

Mstn gene, Egyptian buffalo, PCR, Sequencing, SNPs

Authors

First Name

Michel

Last Name

Saad

MiddleName

Fahmy

Affiliation

Department of Physiology, Fac. of Vet. Med., Kafrelsheikh Univ.

Email

-

City

-

Orcid

-

First Name

Shawky

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Physiology, Fac. of Vet. Med., Kafrelsheikh Univ.

Email

smahmoud@vet.kfs.edu.eg

City

-

Orcid

0000-0003-2216-8288

First Name

Mohammed

Last Name

Abu El-Magd

MiddleName

-

Affiliation

bDepartment of Anatomy& Embryology, Fac. of Vet. Med., Kafrelsheikh Univ.

Email

mohamed.abouelmagd@vet.kfs.edu.eg

City

-

Orcid

0000-0002-3314-9202

First Name

Rasha

Last Name

Alwakeel

MiddleName

Alsaed

Affiliation

Department of Physiology, Fac. of Vet. Med., Kafrelsheikh Univ.

Email

-

City

-

Orcid

-

Volume

12

Article Issue

1

Related Issue

16456

Issue Date

2014-04-01

Receive Date

2014-01-01

Publish Date

2014-04-01

Page Start

111

Page End

126

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 12_Issue 1_Pages 111-126.pdf

PDF . 504.7kB

Link

https://kvmj.journals.ekb.eg/article_110132.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110132

Order

7

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

ASSOCIATION BETWEEN A31GSNP OF THE MYOSTATIN GENE AND SERUM LEVELS OF ALP, GOT, P AND CA IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)

Details

Type

Article

Created At

22 Jan 2023