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110129

LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

Leptin plays an important role in the regulation of development of the mammary gland and its polymorphisms were shown to be associated with milk quantity and quality in cattle. However, no SNP was detected and no association studies was conducted on leptin gene in Egyptian buffalo. Therefore, the objective of this study was to detect leptin gene polymorphisms and to determine associations between these polymorphisms and milk traits in Egyptian water buffalo. A PCR product of 400bp, including intron 2 of leptin gene was amplified using PCR and subsequently, subjected to sequence analysis to identify its different allelic patterns. Two novel SNPs,G247T at nucleotide number 963 and T282G at nucleotide number 998, were detected in LEP intron 2 in Egyptian buffaloes. The associations analysis revealed no significance association between these SNPs and milk traits(P>0.05) in all examined animals. Consequently, our results indicated that the LEPG247T and T282GSNPs does not influence milk yield or milk composition in Egyptian buffalo and these SNPs could not be used in a marker assisted selection program to improve milk traits in Egyptian buffalo

DOI

10.21608/kvmj.2014.110129

Keywords

leptin, Egyptian buffalo, PCR, Sequencing, SNPs, Milk Traits

Authors

First Name

Safaa

Last Name

Abdo

MiddleName

ELsayed

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

Mohammed

Last Name

Abu El-Magd

MiddleName

-

Affiliation

Department of Anatomy& Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

mohamed.abouelmagd@vet.kfs.edu.eg

City

-

Orcid

0000-0002-3314-9202

First Name

Abeer

Last Name

ElNahas

MiddleName

Fikry

Affiliation

Department of Animal Husbandry and Wealth development, Faculty of Veterinary Medicine, Alexandria University

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

Mansour

MiddleName

Abd El-Kader

Affiliation

Department of Anatomy& Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

Volume

12

Article Issue

1

Related Issue

16456

Issue Date

2014-04-01

Receive Date

2014-01-01

Publish Date

2014-04-01

Page Start

97

Page End

110

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 12_Issue 1_Pages 97-110.pdf

PDF . 479.2kB

Link

https://kvmj.journals.ekb.eg/article_110129.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110129

Order

6

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)

Details

Type

Article

Created At

22 Jan 2023