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109536

ISOLATION AND IDENTIFICATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) FROM POULTRY MEAT AND POULTRY PRODUCTS

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

A total of 500 different samples were collected from poultry meat, poultry products and their human contacts in abattoirs from different Egyptian Governorates (Damietta,Dakahlia,Giza and Cairo) in 2012 and 2013. the Samples were tested for presence ofS. aureusby isolation and biochemical identification, the results revealed that coagulase positive S. aureuswas present in83 samples (61.03%).The isolated strains of coagulase positive S.aureus were tested for antimicrobial sensitivity. Most strains were resistant to penicillin and ampicillin, less resistance was appeared to amoxicillin, oxacillin, Methicillin, and ceftriaxone, while all strains were sensitive to, amoxicillin clavulinic acid and vancomycin. Using PCR technique, amplification of 310 bp fragment of mecA gene from the extracted DNA of all isolated coagulase positive S.aureus strains isolated from different chicken samples and human contacts resulted in 5 samples from muscles and skin were positive, Using sequencing technique tomec A gene in  three positive strains, the Phylogenetic analysis of mecA gene of these isolates were clustered together and little away from other published isolates of MRSA, Amino acid  identities were 99.5% among the analyzed isolates, The three isolates shared 87.2%-89.2% identity with other Staphylococcus aureus isolates.

DOI

10.21608/kvmj.2015.109536

Keywords

Methicillin, Resistant Staphylococcus Aureus

Authors

First Name

Salwa M.

Last Name

Helmy

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Dept. Faculty of Veterinary Medicine Kafrelsheikh University, Egypt.

Email

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City

-

Orcid

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First Name

Soad A.

Last Name

Nasef

MiddleName

-

Affiliation

Central Laboratory for Veterinary Quality Control on Poultry production, Animal health research institute, Dokki, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Etab M.

Last Name

AboRemela

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Dept. Fac. of Vet. Med. Kafrelsheikh Univ.,Egypt.

Email

-

City

-

Orcid

-

First Name

Ahmed H.

Last Name

Ramadan

MiddleName

-

Affiliation

Animal health research institute, Mansoura, Dakahlia Egypt

Email

-

City

-

Orcid

-

Volume

13

Article Issue

1

Related Issue

16443

Issue Date

2015-04-01

Receive Date

2015-01-01

Publish Date

2015-04-01

Page Start

57

Page End

69

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 13_Issue 1_Pages 57-69.pdf

PDF . 860.6kB

Link

https://kvmj.journals.ekb.eg/article_109536.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=109536

Order

4

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

ISOLATION AND IDENTIFICATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) FROM POULTRY MEAT AND POULTRY PRODUCTS

Details

Type

Article

Created At

22 Jan 2023