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109535

IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

UL45 was one of the tegument proteins that have been identified in both EHV-1 and EHV-4, it was recorded that ORF 15 EHV-1was homologues to UL45 (HSV). Until now, the function of UL45 protein in cell culture and animal models is poorly understood. To investigate the function of the UL45 protein, we expressed the UL45 gene in prokaryotic expression vector pGEX-6P-1 to express UL45 protein and used the resulting protein to raise guinea pig antiserum. In western blotting experiments, the antiserum directed against the N-terminal of EHV-1 UL45 protein specifically recognized a protein with molecular weight ranging from 30 to 60 kDa in the infected cells lysates. The immunofluorescence assay also showed that EHV-1 ORF15 antiserum was able to probe the UL45 protein which is localized mainly in the cytoplasm associating with the membrane of the cells infected with EHV-1 Ab4p.

DOI

10.21608/kvmj.2015.109535

Keywords

Equine herpes virus -1 (EHV-1), UL45 protein, protein expression, Polyclonal antibody, Immunofluorescence assay

Authors

First Name

Samy

Last Name

Kasem

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt

Email

samykasem2@gmail.com

City

-

Orcid

-

First Name

Gabr

Last Name

Albagory

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

Abdelkader

MiddleName

-

Affiliation

Department of Anatomy, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt

Email

-

City

-

Orcid

-

First Name

Hideto

Last Name

Fukushi

MiddleName

-

Affiliation

Department of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan

Email

-

City

-

Orcid

-

First Name

Noura

Last Name

Alkhalefa

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Nayel

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City University, Sadat City, Egypt

Email

-

City

-

Orcid

-

First Name

Tarek

Last Name

Kamal

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt

Email

-

City

-

Orcid

-

First Name

Al-Said

Last Name

Dawod

MiddleName

-

Affiliation

Animal research institute, Kafrelsheikh branch, El Geish Street, Kafrelsheikh, Egyp

Email

-

City

-

Orcid

-

Volume

13

Article Issue

1

Related Issue

16443

Issue Date

2015-04-01

Receive Date

2015-01-01

Publish Date

2015-04-01

Page Start

35

Page End

55

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 13_Issue 1_Pages 35-55.pdf

PDF . 931.8kB

Link

https://kvmj.journals.ekb.eg/article_109535.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=109535

Order

3

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1

Details

Type

Article

Created At

22 Jan 2023