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109849

ISOLATION AND IDENTIFICATION OF CANINE ADENOVIRUS TYPE 2 FROM THE EGYPTIAN DOGS

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

Canine infectious tracheobronchitis (kennel cough) caused by CAV-2 is one of the most prevalent respiratory infections of dogs. Kennel cough characterized by frequent dry and hacking coughing high morbidity but low mortality rate. In this study, nasal and ocular discharge samples have been taken from 59 dogs from the veterinary clinics of pet animals in Cairo, Egypt of different age and sex from 2013 to 2014. It showed clinical symptoms, a systemic disease which characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Isolated virus was identified by qualitative chromatographic immunoassay by using Anigen Rapid CIRD-3 Ag Test Kit, the result was positive for CAV in only 7 samples. Then the virus from positive samples was passaged for 3 blind passages in Vero cell then subjected to FAT and haemagglutination tests, the results indicated that the virus was most likely to be present in the respiratory tract. The virus was processed and inoculated onto confluent monolayers of MDCK, Vero, BHK21, and MDBK cells for 10 passages using standard virological techniques. The Cytopathic effect (CPE) appeared and MDCK cells were the most susceptible cell culture where they gave the highest infectivity titre.

DOI

10.21608/kvmj.2015.109849

Keywords

Canine Adenovirus, MDCK, VERO, MDBK, BHK21, cPE, kennel cough, fat

Authors

First Name

S. G.

Last Name

Kasem

MiddleName

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Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

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City

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Orcid

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First Name

A. M.

Last Name

Kotb

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute. Abbassia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

N. F

Last Name

Al-Maria

MiddleName

-

Affiliation

Dept. of Agric. Botany, EPCRS Excellence Center, Fac. of Agric. Kafrelsheikh

Email

-

City

-

Orcid

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Volume

13

Article Issue

2

Related Issue

16442

Issue Date

2015-10-01

Receive Date

2015-07-01

Publish Date

2015-10-01

Page Start

115

Page End

127

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 13_Issue 2_Pages 115-127.pdf

PDF . 713.6kB

Link

https://kvmj.journals.ekb.eg/article_109849.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=109849

Order

8

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

ISOLATION AND IDENTIFICATION OF CANINE ADENOVIRUS TYPE 2 FROM THE EGYPTIAN DOGS

Details

Type

Article

Created At

22 Jan 2023