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109790

EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Veterinary Surgery and Theriogenology.

Abstract

The effect of supplementing TCM-199 culture medium with bovine sera on buffalo's oocytes maturation in vitro and expression of  some related genes were studied. A total 1149 immature buffalo oocytes were randomly divided into three groups, G1( n=315) were cultured in medium with no serum supplement(control), G2( n=531) were cultured in medium supplemented with 10% Fetal calf serum (FCS) and G3(n= 303) were cultured in medium supplemented with 10% Estrous cow serum (ECS). After maturation, oocytes in each group were classified according to its shape and expansion into 2 subgroups, good (GO) and fair (FO) expanded oocytes. Expression of GAPDH, CX43, IGF II and β- Actin genes were studied in each subgroup. Results revealed that G3 and G2 showed high maturation rate (P

DOI

10.21608/kvmj.2015.109790

Keywords

Buffalo oocytes, IVM, Gene expression, type of serum

Authors

First Name

Sh.M.

Last Name

Shamiah

MiddleName

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Affiliation

Animal Production Research Institute, Agriculture Research Center, Egypt

Email

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City

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Orcid

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First Name

A. K.

Last Name

Kadoom

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Egypt.

Email

-

City

-

Orcid

-

First Name

A. F.

Last Name

Ashour

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Egypt.

Email

-

City

-

Orcid

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Volume

13

Article Issue

2

Related Issue

16442

Issue Date

2015-10-01

Receive Date

2015-07-01

Publish Date

2015-10-01

Page Start

67

Page End

82

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 13_Issue 2_Pages 67-82.pdf

PDF . 707.9kB

Link

https://kvmj.journals.ekb.eg/article_109790.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=109790

Order

5

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES

Details

Type

Article

Created At

22 Jan 2023