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109773

ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

Foot and mouth disease virus (FMDV) is the most economically important veterinary pathogen due to its highly infectious nature and ability to cause persistent infection.In the period betweenAugust to October2014, an outbreak ofFoot and Mouth Disease(FMD) occurred in Egypt. This study was designated to isolate and detect the current strains of FMDV circulating in Egypt. Twenty sixtissue samples were collected from clinically diseased cattle and buffaloes from Kafrelsheikh province.The isolation of the causative agent wasdone by inoculation of baby mice intraperitoneally then passed to Baby Hamster Kidney-21 (BHK-21) cells. The inoculated mice showed paralysis and death, while the infected BHK-21 showed clear CPE (cell rounding and cell death). Molecular characterizations of the FMDV were doneusing universal primers followed by serotype specific primers for A, O, C and SAT2. Only serotypes SAT2 and A were detected with an overall detection rate of 53.8%.Results of phylogenetic tree of the current FMDV strains revealed that there is no significant divergence among the recent isolated strains and other Egyptian isolated strains from different localities.

DOI

10.21608/kvmj.2015.109773

Keywords

FMDV, Isolation And Molecular Characterization

Authors

First Name

Samy

Last Name

Kasem

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

samykasem2@gmail.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Magouz

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

G. Fekry

Last Name

EL- Bagoury

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Banha University

Email

-

City

-

Orcid

-

First Name

Magdy

Last Name

Abdelaty

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Abeer

Last Name

Zaghlol

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

Volume

13

Article Issue

2

Related Issue

16442

Issue Date

2015-10-01

Receive Date

2015-07-01

Publish Date

2015-10-01

Page Start

17

Page End

35

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 13_Issue 2_Pages 17-35.pdf

PDF . 815.9kB

Link

https://kvmj.journals.ekb.eg/article_109773.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=109773

Order

2

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014

Details

Type

Article

Created At

22 Jan 2023