600 samples were collected from diarrheic broiler chickens and from
apparently normal chickens in Fayoum Governorate .The bacteriological
examination revealed that a total of 530 bacterial isolates were
recovered from the 600 broiler chickens under examination. Cloacal
swabs (130), cecal contents (350), unabsorbed yolk sac (40), liver and
gall bladder (40) and heart blood (40).
Concerning the type of isolated bacteria from broiler chickens E. coli
was the predominate (59.3) %. 53.3%of isolates were from diarrheic
birds and 6.0% from apparently healthy chickens, followed by Proteus
mirabilis (14.8%). Out of them were 11.6% from diarrheic birds and
3.2% from apparently healthy birds. And Proteus vulgaris (3.3%). Out
of them were 2.5% from diarrheic broiler chickens and 0.8% from
apparently healthy one.
E. coli was the most predominant bacteria recovered from the
examined cases. 50 isolates of E. coli which were isolated from large
intestines tested for in vitro pathogenicity using Congo red dye. The
result showed fundamental variation for the growth of E. coli of
diarrheic and apparently healthy origin on Cango red dye as 86% of
E. coli isolated from chickens gave red colonies (pathogenic) while
16% of E. coli isolates did not bind to Congo red dye gave white
colonies (non pathogenic). Eight E. coli isolates recovered from examined diseased broiler
chickens were serotyped and revealed the following : 3 isolates O114
K90, 2 isolates O26 K60, 2 isolates and one isolate O91 K - .
Ten Salmonella spp. isolates recovered from examined diseased broiler
chickens were serotyped and revealed that 6 isolates were belonging
to Salmonella Enteritidis and 4 isolates belonged to Salmonella Virchow.
Aeromonas hydrophila, Salmonella Enteritidis, Salmonella Virchow
and E. coli were examined for antibiotic sensitivity test. It was found
that Aeromonas hydrophila isolates were sensitive to gentamycin,
doxycycline, norfloxacin, enrofloxacin, chloramphenicol and ciprofloxacin,
Salmonella Enteritidis isolates were sensitive to chloromphenicol,
enroloxacin, norfloxacin, colistin, ciprofloxacin and Gentamycin,
neomycin and doxycycline, but Salmonella Virchow isolates were
sensitive to chloromphenicol, enrofbxacin, norlooxacin, colistin, neomycin,
ciprofloxacin, gentamicin, cephalexin and doxycycline, while E. coli
isolates were sensitive to gentamicin, doxycycline and norfloxacin,
chloramphenicol, cephalexin, norfloxacin and colistin.