This investigation was carried out to determine the incidence of bacterial respiratory pathogens in cattle. A total 300 samples were collected from the respiratory system of 200 sample of 100 slaughtered cattle, 50 apparently healthy cattle and 50 animals with respiratory affections. Bacteriological examination of these samples from recent slaughter and living cattle resulted in isolation of 150 (65.22%) and 80 (37.78%), respectively. The results showed that 230 animals were positive with an incidence of (76.67%), of which some cases showed mixed infection and few had one bacterial isolate in a pure form in culture. The total numbers of isolates were 214 of which 70 (28.09%). Staphylococcus spp. 90 (42.06%), E. coli, 25 (11.66%) Proteus spp. 15 (7.01%), Pasteurealla spp., 9 (4.21%), Citrobacter spp. and 15 (7%) Pseudomonas spp. Besides, the antibiotic sensitivity test was done in vitro on some pathogenic respiratory bacterial isolates. Most microorganism were sensitive to enerofloxacin.