Background: The quality of life of medical students could be affected by many educational stressors and improving it may have a positive effect on their physical, mental health and their academic achievements. Objectives: To assess the quality of life (QOL) of medical students at Faculty of Medicine, Tanta University, at the six educational levels and the most important factors related to their quality of life. Methods: A cross sectional study was conducted on 431 medical students at Faculty of Medicine, Tanta University, Egypt, during the academic year 2016/ 2017. A multistage random sampling technique was performed. The WHO Quality of Life Health Survey- Short Form SF-36 was used. Results: Regarding Quality of Life SF-36; medical students had good quality mean score regarding the role emotional limitation domain (85.9±28.8) and physical functioning domain (66.1± 22.9). There were no significant difference between the six grades regarding physical health component, the mental health component and the total quality of life scores. Lower quality of life score were confined to female students with rural background and lack of sleep and physical activity (p <0.05). Conclusions:  The medical students