The current study aimed to determine the impact of using silver nanoparticles (SNaPs) or graphene nanoparticles (GNaPs) as a water supplementation in broiler chicken on productive performance and microbial load in small intestine and ceca of broiler chicken at thirty five days of age. The study comprised of 270 unsexed broiler chicks (Cobb 500) one-day old, which were divided randomly into nine experimental treatments in 3 replicates for each treatment. Four levels of each types (SNaPs or GNaPs) of nanoparticles (2.5, 5, 7.5 and 10 ppm/ L) were used as well as control group. At thirty five days of age, four birds from each treatment were slaughtered to obtain intestinal content samples. Results showed that live body weight (LBW) and body weight gain (BWG) were differed significantly by different levels and different types of nanoparticles. However, LBW, BWG  feed consumption (FC) were significantly higher in birds received drinking water supplemented with GNaPs than those received SNaPs, but they were not affected by different levels of nanoparticles. Also, feed conversion ratio (FCR) was not affected by neither types nor levels of nanoparticles. On the other hand, a bacterial count in small intestine was affected significantly by both types and levels of nanoparticles. Also bacterial counts in ceca was affected significantly by both types and levels of nanoparticles Lactobacillus spp. and E. Coli counts were decreased in small intestine and ceca with increasing nanoparticles levels in drinking water. However, the birds received drinking water supplemented with SNaPs, recorded significantly higher Lactobacillus spp. and E.Coli counts than those received GNaPs in both small intestine and ceca. It can be concluded that GNaPs have highest effects than SNaPs on productive performance and microorganism in small intestine and ceca.