This study was designed to investigate the effect of diets inoculated with antifungal metabolites of Lactobacillus Plantarum DSMZ 20191 on growth performance and quality parameters of the Nile Tilapia fingerlings (Oreochromis niloticus).  Fish diets; animal, plant and mixed were sprayed with the antifungal metabolites levels of (1, 2, 3, 4 and 5 ml/100g for each diet); packed using polyethylene bags and stored under room temperature for 90 days. Tilapia fingerlings were fed on diets inoculated with 5% of the metabolites extract for 180 days. Water quality, growth performance (GP) parameters and quality criteria of Tilapia treatments were determined. Results showed that L. Plantarume specially at 5% had a high activity against molds contaminated animal fish diet, moderate in plant and low in mixed diets. Also, the growth promoters (probiotic) improved GP and reduced mortality rate, mixed and plant diets increased weight gain (WG) while animal diet gave the best protein efficiency ratio (PER).Concerning quality criteria, the metabolites of Lb. plantarum could slightly decrease the pH value in fish fed on treated diets compared with control sample, while total volatile bases (TVB), trimethylamine (TMA) and thiobarbituric acid (TBA) values of fish fed on mixed protein diet were lowered compared with the control sample. Finally, it could be concluded that the inoculated fish diets by metabolites improved the growth rate, chemical composition and quality criteria of Tilapia fish flesh compared with control sample and this leads to extend shelf-life for these fish.