A trial was carried out to determine the effects of diet supplementation with propolis on immunological status and bone physicochemical properties of broiler at 6 weeks of age. Two hundred and forty one-day-old unsexed Cobb broilers chicks were randomly distributed into four equal experimental groups; with three replicates of 20 chicks each. They were fed on one of the following propolis levels; 0, 125, 250 and 500 mg/kg diet. The tibia length, percentage relative to live body weight, dry matter, ash of tibia bone, phosphorus and calcium concentrations were determined at the end of the experiment. The results showed that, propolis supplementation at 125, 250 and 500 mg/kg diet increased (p<0.05) the tibia length by 3, 2.4 and 3.4%, respectively, when compared to the control group. The higher value of tibia percentage relative to live body weight was recorded in the group fed propolis supplementation at 125 mg/kg diet, followed by groups fed 250, 500 mg propolis/kg diet and group that fed control diet. Propolis supplementation at 125, 250 and 500 mg/kg increased phosphorus concentrations by 4.6, 1.1 and 1.8 %, respectively. Also, calcium concentrations in ash were increased by 0.6, 2.9 and 3.1 %, respectively, when compared to the control group. Although, all groups had haemagglutination inhibiting antibodies against NDV, the highest haemagglutination inhibiting antibody titer was obtained from birds fed diets supplemented with 125 and 250 mg propolis/ kg diet at 35 days of age. In addition, data showed that the concentration of 500 mg propolis/kg diet did not significantly influence the humoral immune response of broiler against NDV.