This study was performed in Baghdad city within 5 different regions for buffalo aggregation ,which contained Al-Fadhlyia ,Alma'mal, Abu- Ghruib, Al-Taji and Boob Al Sham through the years 2012 to 2013 ,randomly data collected from buffalo and their numbers are 139 and the total numder of animal was 9252 head ,and holding size ranged 5 to 500 head /breeder.They was used SAS program for statistical analyses for calf mortality less than 1 years and between 1 to 2 years and they are study the effect of sex of animal and region in percentage of mortality. There was a highly significant effect was studied (P<0.01) for sex of calf in rate of calf losses for male and female in all studied area, and the highest percentage for calf losses in male and female were in Al- Fadhlyia region (36.23 and 37.17%), respectively and the lowest mortality were in Boob Al-Sham region (7.09 and 5.60 %) respectively while the effect of region did not found any significant effect except in Abu-Ghruib region (P<0.05) and female calf losses was twice than male 20 .61 and 12.87%, respectively. The calf mortality within 1 to 2 years was the lack of significance in the effect of region on died animals for male and female except two regions Al –Fadhlyia and Abu-Ghruib (P<0.05) but the effect of sex of calf had a significant effect (P<0.01) in both sex ,the highest percentage for mortality was recorded in Al-Fadhlyia region (28.06 and 38.07%) for male and female respectively ,but they observed lowest mortality in Boob Al-Sham which was 7.12 and 7.54 % in male and female, respectively. Because of the difficulty to obtain the information from buffalo breeders for causes of death and the age for died animals and age of dam (parities) and calving season, so they need to build a research station that specialized for buffalo breeding (rearing) and using correct information for causes of death and to supply animal treatments & vaccination.