The aim of this study was to determine the influence of different sources of carbon with and without addition of probiotics on growth performance, and feed utilization of Indian white shrimp (Fenneropenaeus indicus), in addition to water quality of biofloc production system. Control diet (35% CP), in addition to eight treatments based on partial and complete substitution of artificial feeds were used. The biofloc used in the treatments were collected from different reactors with different carbon source (Corn flour and Barley flour) with and without probiotics addition. The experiment lasted for 28 days. At the end of the experiment, the results indicated that there were significant differences between treatments in growth performance and feed utilization. The highest survival was shown by the shrimp fed with barley flour fed biofloc without probiotics and control treatments (100 ± 0.0%), while the lowest survival was shown by the shrimp fed with corn flour fed biofloc with probiotics (54 ± 1.0%). The highest specific growth rate was obtained in the control treatment (1.66 ± 0.02%) while, the shrimp fed with biofloc from barley flour carbon source without probiotics showed almost similar specific growth rate (1.61± 0.02) to that of the control treatment. The biofloc fed shrimp showed promising growth values indicating that the biofloc was sufficient for supporting shrimp growth under commercial farming conditions. Hence, the overall experiments allow concluding that based on its nutritional value; the biofloc offers an alternative strategy for an efficient use of nutrient input in aquaculture systems.