The task of this research is assessing the action of two different fibrolytic enzymes supplementation on milk yield and cheese properties by lactating buffaloes. Three sets had been worked on, everyone had three animals of lactating buffaloes after 3 months of parturition, with 30 days interval. The first one was fed on 50% concentrate feed mixture (CFM), 30% corn silage, 20% rice straw served as control (C). The second and third sets were fed on the same control ration supplemented with Asperozym or Tomoko® at 2 g kg-1 DM served as (T1 andT2). These three different types of lactating milk had been used to manufacture of white soft cheese (WSC). Both of milk and WSC samples were analyzed for some chemical parameters (milk & cheese yield, pH, total solids, total protein, ash contents, free fatty acids and amino acids profile). Furthermore, sensory evaluation of WSC samples had been taken in consideration. Data revealed that supplementation with Asperozym or Tomoko® enzymes had significantly (P<0.05) increased 4% fat-corrected milk and cheese yield. On the other hand, WSC samples with both of fibrolytic enzymes were significantly (P< 0.05) rose in short and long chain fatty acids, while Asperozym cheese samples only were lower in medium chain fatty acids compared to control. Regarding to other chemical composition parameters (TN, TN/SN, TVFAs, tyrosine, tryptophan and pH values) of resulted cheese during storage period, all these parameters were significantly (P<0.05) boosted after 15 days of storage. For amino acids profile, Asrozym and Tomoko were lowered (P<0.05) histidine, threonine, isoleucine, leucine, glycine, alanine and proline but Tomoko enzyme raised (P<0.05) valine, methionine, phenylalanine and serine. Supplementation of fibrolytic enzymes did not manipulate (P>0.05) sensory evaluation of WSC samples. Supplementation of fibrolytic enzymes on buffalo's diet did not significantly affect lactation performance except 4% fat corrected milk, fatty acids and amino acids profile in WSC. Therefore, further study must be conducted on the impact of fibrolytic enzymes on different lactating animals.