Revascularization has been commonly known to be limited to the immature teeth. Owing to its promising results, it was suggested as another treatment option to conventional endodontics in mature teeth. Single visit revascularization is still an area of evaluation and was developed to reduce the drawbacks of multiple visits. This report described the outcomes of single visit revascularization both clinically and radiographically in necrotic permanent mature teeth with chronic apical periodontitis after irrigation activation using sonic disinfection protocol. Twelve cases of permanent mature teeth were recruited into the study. Canals were prepared till file X4 Protaper next system. For disinfection protocol, sonic activation (Endoactivator) was used to agitate sodium hypochlorite (2.5%) for sixty seconds followed by 20 ml of 17% EDTA. The bleeding was then induced and Biodentine was placed over blood clot. Glass ionomer was used as a base then composite as final restoration. Treatment outcome was evaluated for 12 months period. After 12 months of follow-up, all teeth showed the absence of clinical signs and/or symptoms and evidence of periapical healing radiographically. Four teeth regained vitality by the end of 12 month period. Favorable outcome for single visit revascularization procedure when activated with sonic irrigation in mature single-rooted teeth with chronic apical periodontitis.