INTRODUCTION: Treatment of molar furcation defects remains a considerable challenge in clinical practice. Furcation defects treatment can vary according to the type and location of the defects. Regenerative procedures are one of the treatment modalities in furcation involvement. In this study Amnion Chorion membrane(ACM) was used with an alloplast to manage grade II furcation defects. OBJECTIVES The aim of this study was to evaluate clinically and radiographically the effect of ACM augmented with alloplast bone graft in management of grade II furcation involvement. MATERIALS AND METHODS: This study was conducted clinically on fourteen patients with fourteen grade II furcation defects. Patients were divided into two groups: test group: included seven furcation defects that were treated by alloplast bone graft covered by ACM, and control group: including seven furcation defects treated with alloplast bone graft covered by a resorbable collagen membrane. Probing depth (P D), clinical attachment loss(CAL)were measured at baseline,3, and 6 months. Cone beam computed tomography(CBCT)was performed at baseline,6, and9months to evaluate bonefill in the furcation defect. RESULTS: ACM has shown reduction in probing depth and clinical attachment loss, and enhanced bone formation when compared to control group treated with resorbable collagen membrane. CONCLUSIONS: ACM is an effective, easy to handle, safe, and time saving membrane that can be used in periodontal regeneration.