INTRODUCTION: The use of platelet concentrates is recent and its efficiency remains controversial. The mineralized plasmatic matrix (MPM), is the latest innovation of platelet concentrates (i.e. PRP and PRF). A new concept of fabricating growth factors-enriched bone graft matrix has been introduced since 2010. It utilizes altering the centrifugation speed and time to produce much larger, denser and richer fibrin matrix containing growth factors, known as Sticky bone. MPM combines the best of both worlds; its mineral phase qualifies it as an osteoconductive scaffold for bone formation while its platelet concentrate phase grants it an osteoinductive property by the slow release of growth factors. OBJECTIVES: To evaluate the effectiveness of using MPM, compared to the use of bone graft alone, to assess the exact effect of each in enhancing the osteogenic differentiation and bone formation. MATERIALS AND METHODS: Ten healthy adult white Albino New Zealand rabbits, after creating two osseous defects in the right femur in each. The defects (upper and lower) were divided in to two groups according to the material used for filling: Group A (Control): in the upper defect, bone graft only was added in the lateral side of the right femur. Group B (Study): in the lower defect, MPM was addedin the lateral side of the right femur. After 2 &8 weeks respectively postopertively,sacrification of the rabbits was done. RESULTS: Data collected from histologicalresults revealed that MPM enhanced bone formation where increased amount of new bone formation was observed in the study group in relation to the control group. At the end of the experimental period,the defect area was almost filled with mature bone which occupied a greater surface area in the study group. Histomorphometric analysis revealed that there was increase in the mean percentage of bone surface area in the study group in comparison to the control group. This increase was statistically significant in the 8 weeks period. CONCLUSIONS: MPM proved its effeciency in enhancement of osseous regeneration