Introduction: The greatest preventive challenge in dentistry is the control of dental biofilm and consequently avoiding dental caries and gingival inflammation. As an adjunct to the mechanical oral hygiene measures, chemical antimicrobial agents seem to offer great benefits in the control of plaque formation and gingivitis, especially in high risk patients wearing orthodontic appliances. Objective: The aim of the study was to evaluate the effect of Triclosan (0.3%) Colgate Total® on gingivitis and plaque formation in patients wearing fixed orthodontic appliances. Materials and methods: Fifty children wearing fixed orthodontic appliances with an age range of 9 to 13 years were randomly assigned into 2 groups (test and control). The test group received the Colgate Total® toothpaste containing 0.32% sodium fluoride and 0.3% Triclosan. The control group received maxfresh® toothpaste containing 0.22% sodium fluoride. All patients were examined clinically to assess their gingival condition using the Plaque index (PI), Gingival Index (GI), Sulcular Bleeding Index (SBI) at follow up periods of 1, 2 and 3 months. Each group shifted to use the alternative agent after a washout period of 10 days to prevent the carryover effect of Triclosan. Results: Showed that the Triclosan toothpaste users experienced significant reduction in plaque accumulation and gingivitis compared to the fluoride toothpaste users in the first and second follow up periods. The results also showed significant difference compared to the baseline. Conclusion: Triclosan toothpaste proved to have marked antiplaque and antigingivitis effects. Triclosan toothpaste proved to counteract the aggravated gingival effects that can be introduced by the fixed orthodontic appliances in children at the age of puberty