Introduction: Bacteria and their by-products are the main cause of pulpal and periapical diseases. That is why all routes between root canal system and peridontium should be sealed to prevent bacterial leakage. Objectives: Was to compare the coronal bacterial leakage between different obturation materials. Materials and methods: Sixty single-canaled lower premolars were used in this study. Teeth were decoronated to standardize root length to 12 mm. Instrumentation was done using Protaper universal rotary system to file size F4, then teeth were divided into six groups, four experimental and two control (n=10). Group I: obturation was done using CPoint and Endosequence bioceramic sealer using single-cone technique; Group II: obturation was done using Protaper gutta percha and Endosequence bioceramic sealer; Group III: obturation was done using Protaper gutta percha and Adseal resin sealer; Group IV: obturation was done using Protaper gutta percha and MTA Fillapex sealer; Group V: used as positive control; Group VI: used as negative control. Groups II, III, IV, VI were obturated using lateral condensation technique. A split-chamber microbial leakage model was used to detect bacterial leakage. Turbidity in the lower chamber was observed daily for 60 days. Data were analyzed using Kruskal–Wallis and Monte-Carlo test. Results: all experimental groups showed leakage throughout the study period, Group I showed the best results with a mean of (37.30 ± 23.61) days while Group II and III showed nearly equal results with a mean of (32.10 ± 22.57) and (33.10 ± 19.90) days, respectively. Group IV showed a mean of (16.90 ± 8.21) days and was found to be statistically significant than the other groups (P= 0.048). Conclusions: None of the tested materials were able to provide a complete hermetic seal. CPoint with endosequence bioceramic sealer provided the best coronal seal while MTA Fillapex was the worst. CPoint obturation system can be an alternative to lateral condensation technique.