Background: Bleeding in late pregnancy is the most common obstetric emergency. It is serious complication of pregnancy occurring within the third trimester and also a major cause of perinatal maternal morbidity and mortality which needs for a highly skilled health care provider to save the life of the pregnant woman and her unborn fetus. The aim of this study: was to evaluate the effect of educational program on nurses' performance regarding bleeding in late pregnancy. Subjects and method: Setting: The study was carried out at antenatal wards in obstetric and gynecological departments at Main Tanta University Hospital, El-Menshawy Hospital affiliated to the Ministry of Health and population and El-Mabara Hospital affiliated to the Health Insurance. Subjects: All available nurses (48 nurses) who were working in the previously mentioned study settings and were provided care for 144 pregnant women with bleeding in late pregnancy. Tools: Two tools were used for data collection; Tool (I): Structured Interview Schedule. It comprised the following parts: Part 1: Socio-demographic characteristics of nurses and Part 2: Assessment of nurses' Knowledge regarding bleeding in late pregnancy. Tool (II): Nurses' practice regarding bleeding in late pregnancy by using an observation checklist. Results: The total knowledge level of the studied nurses regarding bleeding in late pregnancy was increased immediately and four months after implementation of the program with a significant statistical difference P value=0.0001 compared to pre-program. The total practice level of the studied nurses regarding bleeding in late pregnancy was higher immediately and four months after implementation of the program with a significant statistical difference P value=0.0001 compared to preprogram. Conclusion: The findings of present study revealed that there was a significant improvement of knowledge as well as practice among studied nurses regarding bleeding in late pregnancy immediately after the implementation and four months later post program compared to preprogram. Recommendation: Frequent and schedule in-services training program should be applied for nurses at the hospital regarding management of bleeding in late pregnancy in order to improve nurses' knowledge and practices which will be reflected on improving the quality of health care.