Background: Prostate cancer is increasingly becoming one of the most significant health problems facing men and the commonest cause of cancer-related death in men globally. Thus, screening has immense public health importance. The aim of the study: was to evaluate the effect of educational program on knowledge and commitment of male employees at Tanta University regarding prostate cancer screening. Subjects and Method: Design: A quasi-experimental research design was utilized. Settings: This study was conducted at the faculties of the medical campus (Faculty of Nursing, Medicine, Pharmacy, Dentistry, and Faculty of Science) at Tanta University. Subjects: Systematic random sample of 80 male employees, aged 40 to 60 years old who were free from prostate cancer, and willing to participate in the study were included. Tools: Two tools were used for data collection: Tool (I) A structured interview schedule which consisted of two parts: Part I: bio socio-demographic characteristics of studied employees. Part II: Knowledge about prostate cancer and prostate cancer screening. Tool (II) Commitment to prostate cancer screening. Results: The majority of studied employees had a low level of knowledge pre-program. Immediately after the program intervention most of them had a high level of knowledge. Meanwhile, two-thirds of them had a high level of knowledge one month after the program intervention. More than two-thirds of the studied employees had a low level of commitment to prostate cancer screening tests pre and immediately post-intervention. While less than two-thirds of them retain a high level of commitment one month after the program intervention. Conclusion: There was a significant improvement in the total level of knowledge and commitment to prostate cancer screening. Recommendation: An ongoing effort should be undertaken to raise awareness of the significance of prostate cancer and to eliminate screening barriers.