Background: Negotiation skills are qualities that allow two or more parties to reach a compromise. It includes abilities such as communication, persuasion, planning, and cooperating.  Head nurse who understand the basic principles and skills of negotiation will be ready to deal successfully with conflicting situations and become strong negotiators. Aim: Determine effect of educational program on head nurses' negotiation skills for resolution of conflict among nurses in intensive care units. Method: Include all ICUs of Tanta University Main Hospitals and Emergency Hospital. Subjects: All (N=35) head nurses and nurses (N=300). Tools: Tool I: Head nurses knowledge about negotiation skills for resolution of conflict among nurses' questionnaire. Tool II:Existence of conflict among ICU nurses' questionnaire. Tool III:Head nurses self-assessment about practice of negotiation for resolution of conflict among nurses. Results: High percent (76.3%) of ICU nurses existed high level and 13.7% and 10.0% had moderate and low levels of conflict respectively. Pre- program head nurses 74.3% showed poor level of total knowledge about negotiation skills, changed to 82.9% of them showed good level of knowledge post program. Head nurses 25.7% showed satisfactory level preprogram significantly changed to 88.6% at satisfactory level of total practice of negotiation skills for resolution of conflict among ICU nurses post program. Conclusion: Head nurses knowledge and practice about negotiation skills for resolution of conflict among ICU nurses were at low level at Tanta University Main Hospital and Emergency Hospital. Head nurses knowledge and practice improved significantly after attendance of program sessions. Recommendation: Conduct periodical training programs, workshops and seminars for head nurses to refresh their knowledge, and practice of negotiation skills for resolution of ICU nurses conflict.