Abstract. Evidence-based nursing guideline increases the quality of patient care and closes the gap between research outcomes and practice, Evidence Based Practice (EBP) is the use of current research evidence combined with clinical expertise as well as patient values to formulate sound interventions that ultimately improve the quality of patient care .Aim of the study: was to determine the effect of implementing evidence based nursing Guideline on nurses‟ performance related to care providing for children at Pediatric Intensive Care Unit. A quasi-experimental research design was used. Subjects and Method: A conveniences sampling of 40 nurses. Two tools were used to collect the data: Sociodemographic characteristic, nurses' knowledge of Evidence Based Nursing guideline and nurses' practice of Evidence Based nursing by using observational check list. The results revealed that significant improvement in nursing knowledge and practice after the implementation of Evidence Based nursing guideline. Conclusion most of the nurses have poor knowledge and practice score pre implementation and significant increase post implementation. Recommendation: Nurses should provide knowledge and practice in accessing and appraising research that is relevant to their practice guideline. Besides, it is also crucial that the organizations should provide the facilities and support in implementing evidence-based performance guidelines to optimize child nursing care