Background: Cancer is a group of disease that is characterized by uncontrolled growth of abnormal cells. Chemotherapy is a vital treatment for cancer but in the same time it is associated with serious complications that are need standard of nursing care. Aim of the study: Evaluate the effect of implementing a protocol of nursing care on peripheral venous access complications for patients undergoing chemotherapy. Design and Setting: A quasi-experimental research design was used to collect data from Clinical Oncology and Nuclear Medicine Department of Tanta University Hospital. Subjects: A convenience sampling of (60) patients who were undergoing chemotherapy . They were divided into two equal groups; study and control; each group consisted of (30) patients. Tools: Three tools were used for data collection, Tool (I); Bio- socio-demographic and structured interview schedule evaluate patients' knowledge, Tool (II); Vein quality assessment to assess the quality of vein and Tool (III): Peripheral venous access complications assessment tool. Results: As a result of this study, it was determined that there was highly significant improvement in the total level of patients' knowledge, vein quality, visual infusion phlebitis and there was decrease in the incidence of extravasation among study group at 2nd month and post ending protocol of care compared to control group. Conclusion and Recommendation: Aprotocol of care has positive effect on reducing peripheral venous access complications among chemotherapeutic patients. So it was recommended that oncology nurses should follow protocol of nursing care while administrating chemotherapy for patients to decrease incidence of peripheral venous access complications.