Mechanical ventilation is a complex supportive and life saving therapy for many children with respiratory failure. Optimal outcomes for these children are achieved through the skilled delivery of standardized nursing care include management of the airway and ventilator, physical care, emotional support, pain and anxiety control and prevention of complications. The present study aimed to assess the quality of nursing interventions provided to infants receiving mechanical ventilation. The study was conducted at Pediatric Intensive Care Unit of Tanta University Hospital. It included thirty nurses working in the previously mentioned setting and sixty infants who received mechanical ventilation. Two tools were used for data collection: a structure questionnaire sheet and an observational checklist. The results revealed that, more than half of studied nurses had fair knowledge in relation to their educational level. The majority of them had poor practice in relation to the educational level and years of experience in afternoon shift comparing with morning shift. The study recommended that, in-service training programs and workshops should be conducted for those nurses to improve their knowledge and performance about standard nursing care of mechanically ventilated infants with constructive supervision and follow up by head nurses.