Organizational culture plays a critical role in the success of patient safety initiatives because it has an effect on resource provision, and the development of structures and processes of care render. However, a better understanding of the relationship between organizational culture and patient safety could reveal whether aspects of general organizational culture predispose some hospitals to better safety climate. Thus this study aims to determine organizational culture that promotes patient safety at Main Mansura University Hospital and Mansoura International Specialized Hospital. The study sample consists of all nurses (n=196 nurses) in inpatient units at Main Mansura University Hospital and 188 nurses at Mansoura International Specialized Hospital. Two tools were used for data collection, namely; Organizational Culture Inventory (OCI) and Patient Safety Climate in Healthcare Organizations Questionnaire (PSCHO). The study findings indicated that there is a significant correlation between different organizational culture especially constructive culture and patient safety in both selected hospitals. As most nurses reported that the primary factor that influence patient safety climate was management support patient safety. It was recommended further studies are needed to explore the impact of mix of organizational culture on patient safety climate.