Nephrotic syndrome characteristic by proteinuria, hypoalbuminemia, edema, and hyperlipidemia. Certain complications can arise such as infection, thromboembolism, cardiovascular disease, respiratory distress and acute renal failure. Nurses play an important role in providing nursing care to those children and need continues teaching and training program to enhance their knowledge and improving their practice. The aim of the study was to determine the effect of implementing teaching program about care of children with nephrotic syndrome on nurse's knowledge and practice. Method: The study was conducted at Renal Pediatric Unit and Pediatric Intensive Care Unit of Tanta University Hospital.Method: a quasi-experimental design was utilized in this study Sample all available nurses working in the previously mentioned settings were included (40). Tools: two tools were used to called scoidemgraphic data related to nurses and children as well as Nurses Knowledge about nephrotic syndrome (Tool I) nursing observation checklist to assess nurses practice in providing care to children with nephritic syndrome( Tool II) The results showed that, before preprogram total nurses knowledge scores were poor while as (65%) had good scores immediately after program . The total practice scores of studied nurses before program either poor (40%) or fair (60.0%) .While immediately and one month post program, the total practice scores for all nurses were good. Conclusion: There was statistical significant difference before and after program implementation regarding to nurse's knowledge and their practice. Recommendation: were suggested that, the important of implementing in service training program about care of children with nephrotic syndrome to enhance nurse's knowledge and improve their practice. Written pamphlet or booklet containing information about care of nephrotic syndrome should be available in renal pediatric units.