Aim of the study to compare effects of different maternal positions during non-stress test on maternofetal physiological parameters. Setting: the current study was conducted at fetal monitoring room of obstetrics and gynecology department, Benha University hospital. Sample: A purposive sample of 72 women was recruited according to inclusion criteria. Three tools were used for data collection 1) A structured interview questionnaire. 2) Maternal-fetal physiological parameters sheet. 3) Visual analogue scale of maternal discomfort. Results: There were a highly statistically significant difference in maternal physiological parameters (systolic blood pressure, diastolic blood pressure, and pulse rate) in the three positions during the non-stress test (p=0.000). Number of accelerations and number of fetal movements in left lateral position was significantly higher than supine and semi fowler positions (p=0.030) and (p=0.133) respectively. Mean time taken for first fetal movement, and reactive non-stress test are significantly less in left lateral position compared to supine and semi fowler positions (p=0.000). Almost the studied women showed moderate variability in the three positions. A highly statistically significant difference regarding discomfort during non-stress test the three positions (p=0.000). Conclusion: The left lateral and semi-fowler positions during non-stress test demonstrated favorable maternofetal physiological parameters and women felt more comfortable than in supine position. The study recommended left lateral and semi-fowler positions can be encouraged as alternative positions during non-stress test.