A field experiment was conducted during 2000 / 2001 and 2001 / 2002 growing seasons at the Ismailia Agric. Exp. Station. The effect of 2 kinds of bio-fertilizer [N-fixer (BFI) and P-dissolving bacteria (8E2)1 and three levels of N-fertilizer (30. 60 and 90 kg N/fed.) were studied. The experimental design used was a split plot with three replications. Ni-trogen levels were arranged in the main plots, meanwhile the bio. fertilizers in the sub plots. Results indicated that plant height, spike length, number of spikes/m2, number of grains/spike, straw yield, grain yield as well as crude protein, phosphorus and potassium percentages in barley grains were significantly increased by increasing N rate. When raising N fertiliz-er from 30 to 90 kg/fed, 1000 grains weight was significantly decreased either in both seasons or In combined analysis. Weight of grains/spike was significantly Increased with increasing N fertilizer in the first season, while in combined analysis this character responded only to higher dose of N-fertilizer.
The percentage increase in grain of barley due to raising N-fertilizer rate from 30 to 60, 60 to 90 and 30 to 90/fed. were respec• lively 31%, 30% and 71% for the first season. 20%, 22.6% and 47.4% for the second season and 25%, 26% and 59% for the combined analy. sis. Bio-fertilizer inoculation (BE1) significantly increased the plant height, spike length, number of spikes/m2, number of grains/spike. weight of grains/spike, straw and grain yields compared to non-inoculated control. The percentage increases in grain yield of barley due to (BF1) application were about 28.4%, 17.5% and 22.9% for the first season, second season and their combined analysis, respectively, com-pared to non-inoculated control. On the other hand, most of studied characters were not Influenced by bio-fertilizer (13E2). Moreover, the 1000-kernel weight along with crude protein, phosphorus and potassium percentages in barley grains were not influenced by bio-fertilizer in both seasons and their combined analysis.