This investigation was carried out to develop a model for mo­lecular marker-assisted selection (MAS) to identify the promising culti­vars that posses tolerance to environmental stresses (i.e. drought and salt) at a very early stage of the breeding program. Four cultivars of sugarcane were used in this study, i.e., 686/20, G95/21, 654/9 and F160. The performance of the four cultivars in sand culture experiment revealed that G.86/20 and G95/2 were superior in their salt and drought tolerance according to their performance in some yield- related traits. Cultivar G54/9 was the third, followed by F160, which was very sensitive to the two stresses. Some molecular genetic markers (SDS protein, este­rase and acid phosphatase, isozymes.) were developed for the four culti­vars in relation to the two stresses.