Twenty three fungal species were isolated from different grain sorghum genotypes. Aspergillus nevus, Fusarium oxysporum, F. monilitorme and Gibberella fujikuroi were of the highest oc­currence in the grains of all genotypes examined. Local 29, Selec­tion 1007. Local 162 and Dorado sorghum grain genotypes yielded higher number of fungi than Line C, Giza 15, Local 169 and Local 119, while Local 129. Kuymne, Giza 113, ICSR- 89037 and Giza 54 showed less number of fungi. Grains were inoculated with spores of each of A. flavus, F. moniliforme, F. oxysporum and the results revealed that genotypes Giza 113 and Giza 54 remained essentially free of the three fungi, retained very high level of germinability and showed the lowest mycotoxins accumulation. On the contrary, the highest fungal in­vasion and decline in germinability occurred in inoculated grains and a high mycotoxins production was observed in Local 29, Selec­tion 1007 and Local 162 genotypes. The remaining sorghum gen­otypes showed intermediate susceptibility to fungal invasion and a slight reduction in germinability of inoculated grains, as well as lower mycotoxin formation. Grain coat leachates and grain extracts of thirteen sorghum genotypes exhibited an inhibitory effect on spore germination of A. nevus, F. moniliforme, and F. oxysporum, which varied not only by the genotype, but also depended on fungi tested. High inhibition was obtained by grain coat leachates or grain extracts of genotypes Giza 54 and Giza 113.