The scientific topic of nanobiotechnology is one that is expanding quickly and has potential applications in the life sciences and human healthcare. Nanomedicine and biomedical nanotechnology both heavily rely on silver nanoparticles. Silver is a precious metal that naturally occurs, most frequently as part of a mineral resource in combination with other elements. With regard to various diseases, including bacteria, fungi, viruses, and yeast, silver nanoparticles have a natural antibacterial action. In the current investigation, Duranta erecta leaves were used in the manufacture of silver nanoparticles. The produced silver nanoparticles were studied using UV-VIS spectra, Transmission Electron Microscopy (TEM), X-ray Diffraction (XRD), and Fourier Transfer Infrared Spectra (FTIR). The produced silver nanoparticles from Duranta erecta leaves were tested using the nutrient agar disk diffusion technique by four bacterial species: E. coli, Streptococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. UV-VIS Spectra were utilized to analyze the reduced silver ions and revealed a large resonance band at 410 nm. Through XRD, intense peaks and crystalline nanoparticle production were seen. TEM interpretation revealed spherical AgNPs in a range of diameters from 6 to 16 nm. All these gram-positive(S. aureus and B. subtilis) and gram-negative (E. Coli and P. aeruginosa) bacteria produce positive findings in the current investigation.