Basid:ospores of Pleurotus florida fungus were irradiated in a trial to improve their cultivation capabilities. Spore suspension was irradiated with gamma radiation at dose rates of 0.05, 0.15, 0.25, 0.35, 0.45, 0.55, 0.65, 0.75 and 1.0 kGy. Viable counts of spores decreased as the irradiation dose was increased. Number of survivors was reduced from 1.2 x 10⁷ to 5.5 x 10² CFU/ml, when maximum irradiation dose was used. Different doses of gamma radiation improved radial growth when different sugar sources were used as compared with either non-irradiated culture or tissue culturing of the fungus. Gamma-irradiated culture (IC) showed high yield over both the non-irradiated and tissue cultured fungus and the highest biological efficiency obtained was 139.27 when irradiated at 1.0 kGy. However, Slight effect on yield over the tissue culture was noticed. On the other hand, irradiated culture with 0.25 kGy resulted in increased digestible protein content, which reached 21.363 % as compared with 19.557 and 14.856 % for the non-irradiated and the tissue culture, respectively.